This paper exploits finite element simulations, analytical approaches and experimental data in a study of interference fit fasteners. The influence of both the friction factor and the interference level on the loss of axial load is investigated. An axisymmetric FE model using Abaqus shows that both parameters greatly influence the results. The study of the axial strain enables the most appropriate positions to be identified for sensors intended to investigate the validity of the finite element analysis results. An analytical study is performed to evaluate the relationship between interference level, friction factor and axial strain all along the screw axis. The axial loss of load and the radial pressure between the screw and the plates are also evaluated analytically.