<?xml version="1.0" encoding="utf-8"?>
<TEI xmlns="http://www.tei-c.org/ns/1.0" xmlns:xsi="http://www.w3.org/2001/XMLSchema-instance" xmlns:hal="http://hal.archives-ouvertes.fr/" xmlns:gml="http://www.opengis.net/gml/3.3/" xmlns:gmlce="http://www.opengis.net/gml/3.3/ce" version="1.1" xsi:schemaLocation="http://www.tei-c.org/ns/1.0 http://api.archives-ouvertes.fr/documents/aofr-sword.xsd">
  <teiHeader>
    <fileDesc>
      <titleStmt>
        <title>HAL TEI export of hal-03660265</title>
      </titleStmt>
      <publicationStmt>
        <distributor>CCSD</distributor>
        <availability status="restricted">
          <licence target="https://creativecommons.org/publicdomain/zero/1.0/">CC0 1.0 - Universal</licence>
        </availability>
        <date when="2026-05-22T00:26:49+02:00"/>
      </publicationStmt>
      <sourceDesc>
        <p part="N">HAL API Platform</p>
      </sourceDesc>
    </fileDesc>
  </teiHeader>
  <text>
    <body>
      <listBibl>
        <biblFull>
          <titleStmt>
            <title xml:lang="en">GC/MS-based 13C metabolic flux analysis resolves the parallel and cyclic photomixotrophic metabolism of Synechocystis sp. PCC 6803 and selected deletion mutants including the Entner-Doudoroff and phosphoketolase pathways</title>
            <author role="aut">
              <persName>
                <forename type="first">Dennis</forename>
                <surname>Schulze</surname>
              </persName>
              <idno type="halauthorid">1431454-0</idno>
              <affiliation ref="#struct-92733"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Michael</forename>
                <surname>Kohlstedt</surname>
              </persName>
              <idno type="halauthorid">2259579-0</idno>
              <affiliation ref="#struct-92733"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Judith</forename>
                <surname>Becker</surname>
              </persName>
              <idno type="halauthorid">1431453-0</idno>
              <affiliation ref="#struct-92733"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Edern</forename>
                <surname>Cahoreau</surname>
              </persName>
              <email type="md5">25639dc83b259552dcc1f54c7429c207</email>
              <email type="domain">insa-toulouse.fr</email>
              <idno type="idhal" notation="string">edern-cahoreau</idno>
              <idno type="idhal" notation="numeric">761327</idno>
              <idno type="halauthorid" notation="string">993094-761327</idno>
              <idno type="ORCID">https://orcid.org/0000-0001-8637-0448</idno>
              <affiliation ref="#struct-1002194"/>
              <affiliation ref="#struct-1005814"/>
              <affiliation ref="#struct-1062050"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Lindsay</forename>
                <surname>Peyriga</surname>
              </persName>
              <email type="md5">24d9a2bfd7f6d36164a227dc65475842</email>
              <email type="domain">insa-toulouse.fr</email>
              <idno type="idhal" notation="string">lindsay-peyriga</idno>
              <idno type="idhal" notation="numeric">1198247</idno>
              <idno type="halauthorid" notation="string">875973-1198247</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-6138-7961</idno>
              <affiliation ref="#struct-1002194"/>
              <affiliation ref="#struct-1005814"/>
              <affiliation ref="#struct-1062050"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Alexander</forename>
                <surname>Makowka</surname>
              </persName>
              <idno type="halauthorid">2496029-0</idno>
              <affiliation ref="#struct-462123"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Sarah</forename>
                <surname>Hildebrandt</surname>
              </persName>
              <idno type="halauthorid">2496030-0</idno>
              <affiliation ref="#struct-462123"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Kirstin</forename>
                <surname>Gutekunst</surname>
              </persName>
              <idno type="halauthorid">2496031-0</idno>
              <affiliation ref="#struct-462123"/>
            </author>
            <author role="aut">
              <persName>
                <forename type="first">Jean-Charles</forename>
                <surname>Portais</surname>
              </persName>
              <email type="md5">8ee130aca7a8eb07f528f71680788f01</email>
              <email type="domain">insa-toulouse.fr</email>
              <idno type="idhal" notation="numeric">1097795</idno>
              <idno type="halauthorid" notation="string">875975-1097795</idno>
              <idno type="ORCID">https://orcid.org/0000-0002-3480-0933</idno>
              <idno type="IDREF">https://www.idref.fr/058637397</idno>
              <affiliation ref="#struct-1002194"/>
              <affiliation ref="#struct-1005814"/>
              <affiliation ref="#struct-1062050"/>
            </author>
            <author role="crp">
              <persName>
                <forename type="first">Christoph</forename>
                <surname>Wittmann</surname>
              </persName>
              <email type="md5">20710188a96c42cc51b8e758e931b911</email>
              <email type="domain">uni-saarland.de</email>
              <idno type="idhal" notation="numeric">1037059</idno>
              <idno type="halauthorid" notation="string">994167-1037059</idno>
              <affiliation ref="#struct-92733"/>
            </author>
            <editor role="depositor">
              <persName>
                <forename>INSA Toulouse</forename>
                <surname>SCD</surname>
              </persName>
              <email type="md5">d224dab37fab5d2602fb62245a2db870</email>
              <email type="domain">insa-toulouse.fr</email>
            </editor>
            <funder ref="#projanr-50454"/>
            <funder>German Research Foundation (DFG)INST 256/418-1</funder>
          </titleStmt>
          <editionStmt>
            <edition n="v1" type="current">
              <date type="whenSubmitted">2022-05-05 16:34:09</date>
              <date type="whenModified">2026-04-02 15:38:02</date>
              <date type="whenReleased">2022-05-05 16:47:53</date>
              <date type="whenProduced">2022</date>
              <date type="whenEndEmbargoed">2022-05-05</date>
              <ref type="file" target="https://insa-toulouse.hal.science/hal-03660265v1/document">
                <date notBefore="2022-05-05"/>
              </ref>
              <ref type="file" subtype="greenPublisher" n="1" target="https://insa-toulouse.hal.science/hal-03660265v1/file/s12934-022-01790-9.pdf" id="file-3660265-3196312">
                <date notBefore="2022-05-05"/>
              </ref>
              <fs>
                <f name="inra_etatDocument_local" notation="string" n="VE">
                  <string>inra_etatDocument_local_VE</string>
                </f>
                <f name="inra_publicVise_local" notation="string" n="SC">
                  <string>inra_publicVise_local_SC</string>
                </f>
              </fs>
            </edition>
            <respStmt>
              <resp>contributor</resp>
              <name key="581699">
                <persName>
                  <forename>INSA Toulouse</forename>
                  <surname>SCD</surname>
                </persName>
                <email type="md5">d224dab37fab5d2602fb62245a2db870</email>
                <email type="domain">insa-toulouse.fr</email>
              </name>
            </respStmt>
          </editionStmt>
          <publicationStmt>
            <distributor>CCSD</distributor>
            <idno type="halId">hal-03660265</idno>
            <idno type="halUri">https://insa-toulouse.hal.science/hal-03660265</idno>
            <idno type="halBibtex">schulze:hal-03660265</idno>
            <idno type="halRefHtml">&lt;i&gt;Microbial Cell Factories&lt;/i&gt;, 2022, 21 (1), pp.69. &lt;a target="_blank" href="https://dx.doi.org/10.1186/s12934-022-01790-9"&gt;&amp;#x27E8;10.1186/s12934-022-01790-9&amp;#x27E9;&lt;/a&gt;</idno>
            <idno type="halRef">Microbial Cell Factories, 2022, 21 (1), pp.69. &amp;#x27E8;10.1186/s12934-022-01790-9&amp;#x27E9;</idno>
            <availability status="restricted">
              <licence target="https://creativecommons.org/licenses/by/4.0/">CC BY 4.0 - Attribution<ref corresp="#file-3660265-3196312"/></licence>
            </availability>
          </publicationStmt>
          <seriesStmt>
            <idno type="stamp" n="INSERM">INSERM - Institut national de la santé et de la recherche médicale</idno>
            <idno type="stamp" n="UNIV-TLSE3">Université de Toulouse</idno>
            <idno type="stamp" n="CNRS">CNRS - Centre national de la recherche scientifique</idno>
            <idno type="stamp" n="INSA-TOULOUSE">Institut National des Sciences Appliquées de Toulouse</idno>
            <idno type="stamp" n="AGREENIUM">Archive ouverte en agrobiosciences</idno>
            <idno type="stamp" n="INSA-GROUPE">Groupe INSA</idno>
            <idno type="stamp" n="TBI" corresp="INSA-TOULOUSE">Toulouse Biotechnology Institute</idno>
            <idno type="stamp" n="INRAE">Institut National de Recherche en Agriculture, Alimentation et Environnement</idno>
            <idno type="stamp" n="ANR">ANR</idno>
            <idno type="stamp" n="INRAEOCCITANIETOULOUSE" corresp="INRAE">INRAE Occitanie Toulouse</idno>
            <idno type="stamp" n="MICA-UNITES">MICA-Unités </idno>
            <idno type="stamp" n="TOULOUSE-INP">Toulouse - INP</idno>
            <idno type="stamp" n="UNIV-UT3">Université Toulouse 3</idno>
            <idno type="stamp" n="UT3-INP">Université de Toulouse / Toulouse INP</idno>
            <idno type="stamp" n="UT3-TOULOUSEINP">Université de Toulouse / Toulouse INP</idno>
            <idno type="stamp" n="TBI-TECH" corresp="TBI">Pôle Technologique</idno>
            <idno type="stamp" n="METABOHUB">Metabohub : Infrastructure nationale en métabolomique et fluxomique </idno>
            <idno type="stamp" n="RESTORE">RESTORE</idno>
            <idno type="stamp" n="RESEAU-EAU">Réseau "Systèmes Agricoles et Eau"</idno>
            <idno type="stamp" n="ENVT">École Nationale Vétérinaire de Toulouse </idno>
          </seriesStmt>
          <notesStmt>
            <note type="audience" n="2">International</note>
            <note type="popular" n="0">No</note>
            <note type="peer" n="1">Yes</note>
          </notesStmt>
          <sourceDesc>
            <biblStruct>
              <analytic>
                <title xml:lang="en">GC/MS-based 13C metabolic flux analysis resolves the parallel and cyclic photomixotrophic metabolism of Synechocystis sp. PCC 6803 and selected deletion mutants including the Entner-Doudoroff and phosphoketolase pathways</title>
                <author role="aut">
                  <persName>
                    <forename type="first">Dennis</forename>
                    <surname>Schulze</surname>
                  </persName>
                  <idno type="halauthorid">1431454-0</idno>
                  <affiliation ref="#struct-92733"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Michael</forename>
                    <surname>Kohlstedt</surname>
                  </persName>
                  <idno type="halauthorid">2259579-0</idno>
                  <affiliation ref="#struct-92733"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Judith</forename>
                    <surname>Becker</surname>
                  </persName>
                  <idno type="halauthorid">1431453-0</idno>
                  <affiliation ref="#struct-92733"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Edern</forename>
                    <surname>Cahoreau</surname>
                  </persName>
                  <email type="md5">25639dc83b259552dcc1f54c7429c207</email>
                  <email type="domain">insa-toulouse.fr</email>
                  <idno type="idhal" notation="string">edern-cahoreau</idno>
                  <idno type="idhal" notation="numeric">761327</idno>
                  <idno type="halauthorid" notation="string">993094-761327</idno>
                  <idno type="ORCID">https://orcid.org/0000-0001-8637-0448</idno>
                  <affiliation ref="#struct-1002194"/>
                  <affiliation ref="#struct-1005814"/>
                  <affiliation ref="#struct-1062050"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Lindsay</forename>
                    <surname>Peyriga</surname>
                  </persName>
                  <email type="md5">24d9a2bfd7f6d36164a227dc65475842</email>
                  <email type="domain">insa-toulouse.fr</email>
                  <idno type="idhal" notation="string">lindsay-peyriga</idno>
                  <idno type="idhal" notation="numeric">1198247</idno>
                  <idno type="halauthorid" notation="string">875973-1198247</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-6138-7961</idno>
                  <affiliation ref="#struct-1002194"/>
                  <affiliation ref="#struct-1005814"/>
                  <affiliation ref="#struct-1062050"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Alexander</forename>
                    <surname>Makowka</surname>
                  </persName>
                  <idno type="halauthorid">2496029-0</idno>
                  <affiliation ref="#struct-462123"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Sarah</forename>
                    <surname>Hildebrandt</surname>
                  </persName>
                  <idno type="halauthorid">2496030-0</idno>
                  <affiliation ref="#struct-462123"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Kirstin</forename>
                    <surname>Gutekunst</surname>
                  </persName>
                  <idno type="halauthorid">2496031-0</idno>
                  <affiliation ref="#struct-462123"/>
                </author>
                <author role="aut">
                  <persName>
                    <forename type="first">Jean-Charles</forename>
                    <surname>Portais</surname>
                  </persName>
                  <email type="md5">8ee130aca7a8eb07f528f71680788f01</email>
                  <email type="domain">insa-toulouse.fr</email>
                  <idno type="idhal" notation="numeric">1097795</idno>
                  <idno type="halauthorid" notation="string">875975-1097795</idno>
                  <idno type="ORCID">https://orcid.org/0000-0002-3480-0933</idno>
                  <idno type="IDREF">https://www.idref.fr/058637397</idno>
                  <affiliation ref="#struct-1002194"/>
                  <affiliation ref="#struct-1005814"/>
                  <affiliation ref="#struct-1062050"/>
                </author>
                <author role="crp">
                  <persName>
                    <forename type="first">Christoph</forename>
                    <surname>Wittmann</surname>
                  </persName>
                  <email type="md5">20710188a96c42cc51b8e758e931b911</email>
                  <email type="domain">uni-saarland.de</email>
                  <idno type="idhal" notation="numeric">1037059</idno>
                  <idno type="halauthorid" notation="string">994167-1037059</idno>
                  <affiliation ref="#struct-92733"/>
                </author>
              </analytic>
              <monogr>
                <idno type="halJournalId" status="VALID">1755</idno>
                <idno type="issn">1475-2859</idno>
                <title level="j">Microbial Cell Factories</title>
                <imprint>
                  <publisher>BioMed Central</publisher>
                  <biblScope unit="volume">21</biblScope>
                  <biblScope unit="issue">1</biblScope>
                  <biblScope unit="pp">69</biblScope>
                  <date type="datePub">2022</date>
                  <date type="dateEpub">2022-04-22</date>
                </imprint>
              </monogr>
              <idno type="doi">10.1186/s12934-022-01790-9</idno>
              <idno type="pubmed">35459213</idno>
              <idno type="wos">000786803800002</idno>
              <ref type="publisher">https://microbialcellfactories.biomedcentral.com/articles/10.1186/s12934-022-01790-9</ref>
            </biblStruct>
          </sourceDesc>
          <profileDesc>
            <langUsage>
              <language ident="en">English</language>
            </langUsage>
            <textClass>
              <keywords scheme="author">
                <term xml:lang="en">C metabolic flux analysis</term>
                <term xml:lang="en">Cyanobacteria</term>
                <term xml:lang="en">GC–MS</term>
                <term xml:lang="en">NMR</term>
                <term xml:lang="en">Glycolytic shunt</term>
                <term xml:lang="en">Oxidative pentose phosphate pathway</term>
                <term xml:lang="en">Calvin‑Benson‑Bassham cycle</term>
                <term xml:lang="en">Entner‑Doudoroff pathway</term>
                <term xml:lang="en">Phosphoketolase pathway</term>
                <term xml:lang="en">TCA cycle</term>
                <term xml:lang="en">photomixotrophic growth</term>
                <term xml:lang="en">Glucose</term>
                <term xml:lang="en">CO2</term>
              </keywords>
              <classCode scheme="mesh">Aldehyde-Lyases</classCode>
              <classCode scheme="mesh">Amino Acids</classCode>
              <classCode scheme="mesh">Carbon Dioxide</classCode>
              <classCode scheme="mesh">Gas Chromatography-Mass Spectrometry</classCode>
              <classCode scheme="mesh">Metabolic Flux Analysis</classCode>
              <classCode scheme="halDomain" n="sdv.mp.bac">Life Sciences [q-bio]/Microbiology and Parasitology/Bacteriology</classCode>
              <classCode scheme="halDomain" n="sdv.bbm.bc">Life Sciences [q-bio]/Biochemistry, Molecular Biology/Biochemistry [q-bio.BM]</classCode>
              <classCode scheme="halTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halOldTypology" n="ART">Journal articles</classCode>
              <classCode scheme="halTreeTypology" n="ART">Journal articles</classCode>
            </textClass>
            <abstract xml:lang="en">
              <p>Background Cyanobacteria receive huge interest as green catalysts. While exploiting energy from sunlight, they co-utilize sugar and CO 2 . This photomixotrophic mode enables fast growth and high cell densities, opening perspectives for sustainable biomanufacturing. The model cyanobacterium Synechocystis sp. PCC 6803 possesses a complex architecture of glycolytic routes for glucose breakdown that are intertwined with the CO 2 -fixing Calvin-Benson-Bassham (CBB) cycle. To date, the contribution of these pathways to photomixotrophic metabolism has remained unclear.Results Here, we developed a comprehensive approach for 13 C metabolic flux analysis of Synechocystis sp. PCC 6803 during steady state photomixotrophic growth. Under these conditions, the Entner-Doudoroff (ED) and phosphoketolase (PK) pathways were found inactive but the microbe used the phosphoglucoisomerase (PGI) (63.1%) and the oxidative pentose phosphate pathway (OPP) shunts (9.3%) to fuel the CBB cycle. Mutants that lacked the ED pathway, the PK pathway, or phosphofructokinases were not affected in growth under metabolic steady-state. An ED pathway-deficient mutant ( Δeda ) exhibited an enhanced CBB cycle flux and increased glycogen formation, while the OPP shunt was almost inactive (1.3%). Under fluctuating light, ∆eda showed a growth defect, different to wild type and the other deletion strains.Conclusions The developed approach, based on parallel 13 C tracer studies with GC–MS analysis of amino acids, sugars, and sugar derivatives, optionally adding NMR data from amino acids, is valuable to study fluxes in photomixotrophic microbes to detail. In photomixotrophic cells, PGI and OPP form glycolytic shunts that merge at switch points and result in synergistic fueling of the CBB cycle for maximized CO 2 fixation. However, redirected fluxes in an ED shunt-deficient mutant and the impossibility to delete this shunt in a GAPDH2 knockout mutant, indicate that either minor fluxes (below the resolution limit of 13 C flux analysis) might exist that could provide catalytic amounts of regulatory intermediates or alternatively, that EDA possesses additional so far unknown functions. These ideas require further experiments.</p>
            </abstract>
          </profileDesc>
        </biblFull>
      </listBibl>
    </body>
    <back>
      <listOrg type="structures">
        <org type="institution" xml:id="struct-92733" status="VALID">
          <idno type="ROR">https://ror.org/01jdpyv68</idno>
          <orgName>Universität des Saarlandes [Saarbrücken] = Saarland University [Saarbrücken]</orgName>
          <desc>
            <address>
              <addrLine>Universität des Saarlandes Campus D-66123 Saarbrücken</addrLine>
              <country key="DE"/>
            </address>
            <ref type="url">http://www.uni-saarland.de</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-1002194" status="VALID">
          <idno type="RNSR">196917933G</idno>
          <orgName>Toulouse Biotechnology Institute</orgName>
          <orgName type="acronym">TBI</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <addrLine>INSA / CNRS 5504  UMR INSA /  INRAE 792 135 avenue de Rangueil 31077 Toulouse CEDEX 04</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.toulouse-biotechnology-institute.fr/fr/index.html</ref>
          </desc>
          <listRelation>
            <relation active="#struct-116255" type="direct"/>
            <relation active="#struct-301232" type="indirect"/>
            <relation active="#struct-443875" type="indirect"/>
            <relation name="UMR5504" active="#struct-441569" type="direct"/>
            <relation name="UMR0792" active="#struct-577435" type="direct"/>
          </listRelation>
        </org>
        <org type="researchteam" xml:id="struct-1005814" status="OLD">
          <orgName>MetaToul FluxoMet</orgName>
          <orgName type="acronym">TBI-MetaToul</orgName>
          <date type="start">2019-07-05</date>
          <date type="end">2025-01-01</date>
          <desc>
            <address>
              <addrLine>Plate-forme de Métabolomique et Fluxomique,  TBI/INSAT, UMR5504, UMR792, CNRS, INRA, INSA 135 Avenue de Rangueil, 31077 Toulouse cédex 04, France</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.toulouse-biotechnology-institute.fr/fr/plateformes_technologiques/metabolomique_et_fluxomique.html</ref>
          </desc>
          <listRelation>
            <relation active="#struct-552749" type="direct"/>
            <relation active="#struct-414014" type="indirect"/>
            <relation active="#struct-1002361" type="indirect"/>
            <relation active="#struct-217752" type="indirect"/>
            <relation active="#struct-443875" type="indirect"/>
            <relation active="#struct-300421" type="indirect"/>
            <relation active="#struct-448187" type="indirect"/>
            <relation active="#struct-303623" type="indirect"/>
            <relation name="UAR2039" active="#struct-441569" type="indirect"/>
            <relation active="#struct-577435" type="indirect"/>
            <relation active="#struct-1002194" type="direct"/>
            <relation active="#struct-116255" type="indirect"/>
            <relation active="#struct-301232" type="indirect"/>
            <relation name="UMR5504" active="#struct-441569" type="indirect"/>
            <relation name="UMR0792" active="#struct-577435" type="indirect"/>
          </listRelation>
        </org>
        <org type="laboratory" xml:id="struct-1062050" status="OLD">
          <idno type="IdRef">252908295</idno>
          <idno type="RNSR">202123705E</idno>
          <orgName>Geroscience and rejuvenation research center</orgName>
          <orgName type="acronym">RESTORE</orgName>
          <date type="start">2021-01-01</date>
          <date type="end">2025-01-01</date>
          <desc>
            <address>
              <addrLine>4 bis av. Hubert Curien, Bat INCERE, 31100 TOULOUSE</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-199980" type="direct"/>
            <relation active="#struct-217752" type="direct"/>
            <relation active="#struct-443875" type="indirect"/>
            <relation name="U1301" active="#struct-303623" type="direct"/>
            <relation name="UMR5070" active="#struct-441569" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-462123" status="VALID">
          <idno type="IdRef">027909913</idno>
          <idno type="ISNI">0000000121539986</idno>
          <idno type="ROR">https://ror.org/04v76ef78</idno>
          <orgName>Christian-Albrechts-Universität zu Kiel = Christian-Albrechts University of Kiel = Université Christian-Albrechts de Kiel</orgName>
          <orgName type="acronym">CAU</orgName>
          <desc>
            <address>
              <addrLine>Christian-Albrechts-Platz 4, 24118 Kiel, Germany</addrLine>
              <country key="DE"/>
            </address>
            <ref type="url">http://www.uni-kiel.de/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-116255" status="VALID">
          <idno type="IdRef">026388766</idno>
          <idno type="ISNI">0000 0001 2286 8343</idno>
          <idno type="ROR">https://ror.org/01h8pf755</idno>
          <idno type="Wikidata">Q858979</idno>
          <orgName>Institut National des Sciences Appliquées - Toulouse</orgName>
          <orgName type="acronym">INSA Toulouse</orgName>
          <date type="start">1963-10-21</date>
          <desc>
            <address>
              <addrLine>135, avenue de Rangueil - 31077 Toulouse cedex 4</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.insa-toulouse.fr</ref>
          </desc>
          <listRelation>
            <relation active="#struct-301232" type="direct"/>
            <relation active="#struct-443875" type="direct"/>
          </listRelation>
        </org>
        <org type="regroupinstitution" xml:id="struct-301232" status="VALID">
          <idno type="IdRef">162105150</idno>
          <orgName>Institut National des Sciences Appliquées</orgName>
          <orgName type="acronym">INSA</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-443875" status="VALID">
          <idno type="ROR">https://ror.org/017tgbk05</idno>
          <orgName>Communauté d'universités et établissements de Toulouse</orgName>
          <orgName type="acronym">Comue de Toulouse</orgName>
          <desc>
            <address>
              <addrLine>41 Allée Jules Guesde, 31000 Toulouse</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.univ-toulouse.fr/</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-441569" status="VALID">
          <idno type="IdRef">02636817X</idno>
          <idno type="ISNI">0000000122597504</idno>
          <idno type="ROR">https://ror.org/02feahw73</idno>
          <orgName>Centre National de la Recherche Scientifique</orgName>
          <orgName type="acronym">CNRS</orgName>
          <date type="start">1939-10-19</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.cnrs.fr/</ref>
          </desc>
        </org>
        <org type="institution" xml:id="struct-577435" status="VALID">
          <idno type="ROR">https://ror.org/003vg9w96</idno>
          <orgName>Institut National de Recherche pour l’Agriculture, l’Alimentation et l’Environnement</orgName>
          <orgName type="acronym">INRAE</orgName>
          <date type="start">2020-01-01</date>
          <desc>
            <address>
              <country key="FR"/>
            </address>
          </desc>
        </org>
        <org type="department" xml:id="struct-552749" status="OLD">
          <orgName>MetaboHUB-MetaToul</orgName>
          <date type="end">2025-01-01</date>
          <desc>
            <address>
              <addrLine>Regroupement de 4 plateformes technologiques et d’expertises Toulouse</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www6.toulouse.inrae.fr/metatoul/Presentation</ref>
          </desc>
          <listRelation>
            <relation active="#struct-414014" type="direct"/>
            <relation active="#struct-1002361" type="direct"/>
            <relation active="#struct-217752" type="indirect"/>
            <relation active="#struct-443875" type="indirect"/>
            <relation active="#struct-300421" type="indirect"/>
            <relation active="#struct-448187" type="indirect"/>
            <relation active="#struct-303623" type="indirect"/>
            <relation name="UAR2039" active="#struct-441569" type="indirect"/>
            <relation active="#struct-577435" type="indirect"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-414014" status="VALID">
          <idno type="ROR">https://ror.org/039gscz82</idno>
          <idno type="Wikidata">Q107518509</idno>
          <orgName>MetaboHUB</orgName>
          <date type="start">2013-01-01</date>
          <desc>
            <address>
              <addrLine>135 Avenue de Rangueil,31400 Toulouse, FRANCE</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.metabohub.fr/</ref>
          </desc>
        </org>
        <org type="laboratory" xml:id="struct-1002361" status="OLD">
          <idno type="RNSR">202424518A</idno>
          <orgName>Génopole Toulouse Midi-Pyrénées [Auzeville]</orgName>
          <orgName type="acronym">GENOTOUL</orgName>
          <date type="start">2020-01-01</date>
          <date type="end">2025-01-01</date>
          <desc>
            <address>
              <addrLine>Chemin de Borde-Rouge Auzeville</addrLine>
              <country key="FR"/>
            </address>
          </desc>
          <listRelation>
            <relation active="#struct-217752" type="direct"/>
            <relation active="#struct-443875" type="indirect"/>
            <relation active="#struct-300421" type="direct"/>
            <relation active="#struct-448187" type="direct"/>
            <relation active="#struct-303623" type="direct"/>
            <relation name="UAR2039" active="#struct-441569" type="direct"/>
            <relation active="#struct-577435" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-217752" status="OLD">
          <idno type="IdRef">026404672</idno>
          <idno type="ISNI">0000000121617331</idno>
          <idno type="ROR">https://ror.org/02v6kpv12</idno>
          <idno type="Wikidata">Q1273188</idno>
          <orgName>Université Toulouse III - Paul Sabatier</orgName>
          <orgName type="acronym">UT3</orgName>
          <date type="end">2025-01-01</date>
          <desc>
            <address>
              <addrLine>118 route de Narbonne - 31062 Toulouse</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.univ-tlse3.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-443875" type="direct"/>
          </listRelation>
        </org>
        <org type="regrouplaboratory" xml:id="struct-300421" status="VALID">
          <idno type="ROR">https://ror.org/03m3gzv89</idno>
          <orgName>École nationale vétérinaire de Toulouse</orgName>
          <orgName type="acronym">ENVT</orgName>
          <desc>
            <address>
              <addrLine>23 chemin des Capelles - 31076 Toulouse Cedex</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://envt.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-448187" type="direct"/>
            <relation active="#struct-443875" type="indirect"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-448187" status="VALID">
          <idno type="IdRef">026388820</idno>
          <idno type="ISNI">0000000121766169</idno>
          <idno type="ROR">https://ror.org/033p9g875</idno>
          <orgName>Institut National Polytechnique (Toulouse)</orgName>
          <orgName type="acronym">Toulouse INP</orgName>
          <desc>
            <address>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.inp-toulouse.fr/</ref>
          </desc>
          <listRelation>
            <relation active="#struct-443875" type="direct"/>
          </listRelation>
        </org>
        <org type="institution" xml:id="struct-303623" status="VALID">
          <idno type="IdRef">026388278</idno>
          <idno type="ROR">https://ror.org/02vjkv261</idno>
          <orgName>Institut National de la Santé et de la Recherche Médicale</orgName>
          <orgName type="acronym">INSERM</orgName>
          <desc>
            <address>
              <addrLine>101, rue de Tolbiac, 75013 Paris</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">http://www.inserm.fr</ref>
          </desc>
        </org>
        <org type="regroupinstitution" xml:id="struct-199980" status="VALID">
          <orgName>Établissement Français du Sang [La Plaine Saint-Denis]</orgName>
          <orgName type="acronym">EFS</orgName>
          <date type="start">2000-01-01</date>
          <desc>
            <address>
              <addrLine>20 Avenue du Stade de France 93218 La Plaine Saint Denis</addrLine>
              <country key="FR"/>
            </address>
            <ref type="url">https://www.dondusang.net</ref>
          </desc>
        </org>
      </listOrg>
      <listOrg type="projects">
        <org type="anrProject" xml:id="projanr-50454" status="VALID">
          <idno type="anr">ANR-11-INBS-0010</idno>
          <orgName>METABOHUB</orgName>
          <desc>Développement d'une infrastructure française distribuée pour la métabolomique dédiée à l'innovation</desc>
          <date type="start">2011</date>
        </org>
      </listOrg>
    </back>
  </text>
</TEI>